Cis-Acting Relaxases Guarantee Independent Mobilization of MOB_{Q 4} Plasmids.

ABSTRACT: Plasmids are key vehicles of horizontal gene transfer and contribute greatly to bacterial genome plasticity. In this work, we studied a group of plasmids from enterobacteria that encode phylogenetically related mobilization functions that populate the previously non-described MOB_{Q 4} relaxase family. These plasmids encode two transfer genes: mobA coding for the MOB_{Q 4} relaxase; and mobC, which is non-essential but enhances the plasmid mobilization frequency. The origin of transfer is located between these two divergently transcribed mob genes. We found that MPF_I conjugative plasmids were the most efficient helpers for MOB_{Q 4} conjugative dissemination among clinically relevant enterobacteria. Wh

SUBMITTER: Garcillan-Barcia MP 

PROVIDER: S-EPMC6856555 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature