Establishment and Characterization of a CTC Cell Line from Peripheral Blood of Breast Cancer Patient.

ABSTRACT:

Background

Circulating tumor cell (CTC)-based patient-derived cells are ideal models for investigating the molecular basis of cancer. However, the rarity and heterogeneity of CTCs as well as the difficulties of primary culture limit their practical application. Establishing efficient in vitro culture methods and functionally characterizing CTCs is essential for cancer studies. To this end, we developed an experimental protocol for the isolation, expansion, and identification of breast cancer CTCs.

Methods

The CTC-3 cell line was established from peripheral blood cells of a breast cancer patient. A karyotype analysis was performed. The molecular profile was assessed by flow cytometry, quantitative real-time PCR, and western blot. The characteristics of tumors formed by

SUBMITTER: Zhao P 

PROVIDER: S-EPMC6856591 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature