Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent.

ABSTRACT: The mitochondrial membrane potential (ΔΨ_m ) is the main driver of oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane (IMM), consisting of cristae and inner boundary membranes (IBM), is considered to carry a uniform ΔΨ_m . However, sequestration of OXPHOS components in cristae membranes necessitates a re-examination of the equipotential representation of the IMM. We developed an approach to monitor ΔΨ_m at the resolution of individual cristae. We found that the IMM was divided into segments with distinct ΔΨ_m , corresponding to cristae and IBM. ΔΨ_m was higher at cristae compared to IBM. Treatment with oligomycin increased, whereas FCCP decreased, ΔΨ_m heterogeneity along the IMM. Impairment of cristae structure

SUBMITTER: Wolf DM 

PROVIDER: S-EPMC6856616 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature