Hepatomas are exquisitely sensitive to pharmacologic ascorbate (P-AscH^-).

ABSTRACT: Rationale: Ascorbate is an essential micronutrient known for redox functions at normal physiologic concentrations. In recent decades, pharmacological ascorbate has been found to selectively kill tumour cells. However, the dosing frequency of pharmacologic ascorbate in humans has not yet been defined. Methods: We determined that among five hepatic cell lines, Huh-7 cells were the most sensitive to ascorbate. The effects of high-dose ascorbate on hepatoma were therefore assessed using Huh-7 cells and xenograft tumour mouse model. Results: In Huh-7 cells, ascorbate induced a significant increase in the percentage of cells in the G0/G1 phase, apoptosis and intracellular levels of ROS. High doses of ascorbate (4.0 pmol cell^-1), but not low doses of ascorba

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6857065 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature