Antimicrobial functional divergence of the cecropin antibacterial peptide gene family in Musca domestica.

ABSTRACT:

Background

It has been reported that there are more than ten antimicrobial peptides (AMPs) belonging to the cecropin family in Musca domestica; however, few of them have been identified, and the functions of the other molecules are poorly understood.

Methods

Sequences of the M. domestica cecropin family of genes were cloned from cDNA template, which was reverse-transcribed from total mRNA isolated from third-instar larvae of M. domestica that were challenged with pathogens. Sequence analysis was performed using DNAMAN comprehensive analysis software, and a molecular phylogenetic tree of the cecropin family was constructed using the Neighbour-Joining method in MEGA v.5.0 according to the mature peptide sequences. Antibacterial activity of the synthetic M. domestica cecropin p

SUBMITTER: Peng J 

PROVIDER: S-EPMC6857134 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature