Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins.

ABSTRACT: BACKGROUND:Cerebrospinal fluid (CSF) metabolomic investigations are a powerful tool for studying neurometabolic diseases. We aimed to assess the effect of CSF contamination with blood on the concentrations of selected biomarkers. METHODS:CSF samples were spiked in duplicate with increasing volumes of whole blood under two conditions: (A) pooled CSF spiked with fresh whole blood and frozen to cause red blood cell (RBC) lysis; (B) pooled CSF spiked with fresh blood and centrifuged (the supernatant with no RBCs was frozen until the moment of analysis). CSF concentrations of amino acids, biogenic amines, pterins, and vitamins were analysed by HPLC coupled with tandem mass spectrometry, electrochemical and fluorescence detection. RESULTS:Aspartate, glutamate, taurine, ornithine, glycine, citrul

SUBMITTER: Batllori M 

PROVIDER: S-EPMC6857153 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature