Phenotypic and genotypic characterization of linezolid-resistant Enterococcus faecium from the USA and Pakistan.

ABSTRACT:

Objectives

Linezolid is an important therapeutic option for the treatment of infections caused by VRE. Linezolid is a synthetic antimicrobial and resistance to this antimicrobial agent remains relatively rare. As a result, data on the comparative genomics of linezolid resistance determinants in Enterococcus faecium are relatively sparse.

Methods

To address this knowledge gap in E. faecium, we deployed phenotypic antibiotic susceptibility testing and Illumina WGS on hospital surface (environmental) and clinical isolates from the USA and Pakistan.

Results

We found complete concordance between isolate source country and mechanism of linezolid resistance, with all the US isolates possessing a 23S rRNA gene mutation and the Pakistan isolates harbouring two to three acquire

SUBMITTER: Wardenburg KE 

PROVIDER: S-EPMC6857194 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature