Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom.

ABSTRACT:

Background

Previous glycophylogenetic comparisons of dipteran and lepidopteran species revealed variations in the anionic and zwitterionic modifications of their N-glycans; therefore, we wished to explore whether species- and order-specific glycomic variations would extend to the hymenoptera, which include the honeybee Apis mellifera, an agriculturally- and allergologically-significant social species.

Methods

In this study, we employed an off-line liquid chromatography/mass spectrometry approach, in combination with enzymatic and chemical treatments, to analyse the N-glycans of male honeybee larvae and honeybee venom in order to facilitate definition of isomeric structures.

Results

The neutral larval N-glycome was dominated by oligomannosidic and paucimannosidic struc

SUBMITTER: Hykollari A 

PROVIDER: S-EPMC6858280 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature