Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai'ian lobelioid group.

ABSTRACT:

Premise

Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae).

Methods and results

Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai'ian lobelioid group to determine the broader applicability of the markers.

Conclusions

Results indicate that these primers wi

SUBMITTER: Fant JB 

PROVIDER: S-EPMC6858292 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature