Dataset Information

The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency.

ABSTRACT: The indica and japonica rice (Oryza sativa) subspecies differ in nitrate (NO₃^-) assimilation capacity and nitrogen (N) use efficiency (NUE). Here, we show that a major component of this difference is conferred by allelic variation at OsNR2, a gene encoding a NADH/NADPH-dependent NO₃^- reductase (NR). Selection-driven allelic divergence has resulted in variant indica and japonica OsNR2 alleles encoding structurally distinct OsNR2 proteins, with indica OsNR2 exhibiting greater NR activity. Indica OsNR2 also promotes NO₃^- uptake via feed-forward interaction with OsNRT1.1B, a gene encoding a NO₃^- uptake transporter. These properties enable indica OsNR2 to confer increased effective tiller number, grain yield and NUE on japonica rice, effects enhanced by interaction with an additionally introgressed indica OsNRT1.1B allele. In consequence, indica OsNR2 provides an important breeding resource for the sustainable increases in japonica rice yields necessary for future global food security.

SUBMITTER: Gao Z

PROVIDER: S-EPMC6858341 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency.

Gao Zhenyu Z Wang Yufeng Y Chen Guang G Zhang Anpeng A Yang Shenglong S Shang Lianguang L Wang Danying D Ruan Banpu B Liu Chaolei C Jiang Hongzhen H Dong Guojun G Zhu Li L Hu Jiang J Zhang Guangheng G Zeng Dali D Guo Longbiao L Xu Guohua G Teng Sheng S Harberd Nicholas P NP Qian Qian Q

Nature communications 20191115 1

The indica and japonica rice (Oryza sativa) subspecies differ in nitrate (NO3-) assimilation capacity and nitrogen (N) use efficiency (NUE). Here, we show that a major component of this difference is conferred by allelic variation at OsNR2, a gene encoding a NADH/NADPH-dependent NO3- reductase (NR). Selection-driven allelic divergence has resulted in variant indica and japonica OsNR2 alleles encoding structurally distinct OsNR2 proteins, with indica Os ...[more]

PMID: 31729387

Similar Datasets

Project description:Modern rice cultivation relies heavily upon inorganic nitrogen fertilization. Effective fertilizer management is key to sustainable agricultural development. Field and pot trials were conducted in 2014-2016, including a 15N-labeled urea pot experiment (2014) to investigate mechanism by which optimized nitrogen fertilizer application (OFA) increases nitrogen utilization efficiency (NUE). Results showed that the applied nitrogen recovery efficiencies with OFA were 71.71%, 110.17%, and 51.38% higher than those obtained with traditional nitrogen fertilizer application (TFA) in 2014, 2015, and 2016, respectively. These improvements are attributed mainly to the high recovery efficiency rates derived from spikelet-developing and spikelet-promoting fertilizer applications at the jointing stage and 15-20 d after jointing. Under OFA, the amount of nitrogen fertilizer applied at the early stages was half that used in TFA, which not only promoted the absorption of soil nitrogen, but also reduced nitrogen loss to the environment, as the NUE of basal and tillering fertilizer was only about 22%. Nitrogen applied during the panicle differentiation stage increased the expression of ATM1;1, a NH4 + transporter in roots. This effect significantly improved the uptake of nitrogen derived from fertilizer from jointing to heading stage. Up-regulation of the expression and activity of GS and GOGAT at the panicle differentiation and grain-filling stages promoted nitrogen translocation from vegetative organs to reproductive organs. The uptake of nitrogen derived from fertilizer increased from 22.51% in TFA to 35.58% in OFA. Nevertheless, rice absorbs most of the nitrogen it requires from the soil. The OFA treatment could effectively utilize the environmental compensation effect, promote the absorption and transport of nitrogen, and ultimately lead to improvement in NUE. Future research should aim to understand the soil nitrogen supply capacity in order to apply nitrogenous fertilizer in such a way that it sustains the nitrogen balance.

Dataset Information

The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency.

Publications

The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets