MTORC1 activity is essential for erythropoiesis and B cell lineage commitment.

ABSTRACT: Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase that mediates phosphoinositide-3-kinase (PI3K)/AKT signalling. This pathway is involved in a plethora of cellular functions including protein and lipid synthesis, cell migration, cell proliferation and apoptosis. In this study, we proposed to delineate the role of mTORC1 in haemopoietic lineage commitment using knock out (KO) mouse and cell line models. Mx1-cre and Vav-cre expression systems were used to specifically target Raptor^fl/fl (mTORC1), either in all tissues upon poly(I:C) inoculation, or specifically in haemopoietic stem cells, respectively. Assessment of the role of mTORC1 during the early stages of development in Vav-cre⁺Raptor^fl/fl mice, revealed that these mice do not

SUBMITTER: Malik N 

PROVIDER: S-EPMC6858379 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature