Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules.

ABSTRACT: There are several methods for visualizing purified biomolecules near surfaces. Total-internal reflection fluorescence (TIRF) microscopy is a commonly used method, but has the drawback that it requires fluorescent labeling, which can interfere with the activity of the molecules. Also, photobleaching and photodamage are concerns. In the case of microtubules, we have found that images of similar quality to TIRF can be obtained using interference reflection microscopy (IRM). This suggests that IRM might be a general technique for visualizing the dynamics of large biomolecules and oligomers in vitro. In this paper, we show how a fluorescence microscope can be modified simply to obtain IRM images. IRM is easier and considerably cheaper to implement than other contrast techniques such as differen

SUBMITTER: Mahamdeh M 

PROVIDER: S-EPMC6858481 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature