A Split Transcriptional Repressor That Links Protein Solubility to an Orthogonal Genetic Circuit.

ABSTRACT: Monitoring the aggregation of proteins within the cellular environment is key to investigating the molecular mechanisms underlying the formation of off-pathway protein assemblies associated with the development of disease and testing therapeutic strategies to prevent the accumulation of non-native conformations. It remains challenging, however, to couple protein aggregation events underlying the cellular pathogenesis of a disease to genetic circuits and monitor their progression in a quantitative fashion using synthetic biology tools. To link the aggregation propensity of a target protein to the expression of an easily detectable reporter, we investigated the use of a transcriptional AND gate system based on complementation of a split transcription factor. We first identified two-fragment

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC6858789 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature