A Newly Discovered Drug Resistance Gene rfaF In Helicobacter pylori.

ABSTRACT:

Background

The purpose of this study was to understand the function of rfaF gene in Helicobacter pylori antibiotic resistance.

Methods

The gene homologous recombination method was used for knockout and complementation of H. pylori rfaF gene. Various constructed strains were analysed for drug sensitivity to amoxicillin (AMO), tetracycline (TET), clarithromycin (CLA), metronidazole (MET), levofloxacin (LEV), and chloramphenicol (CHL) by agar plate dilution method. Drug sensitivity was further confirmed using a growth inhibition curve. Ethidium bromide (EB) accumulation experiments were performed to assess cell membrane permeability. PCR and sequence analysis were used to detect the rfaF gene.

Results

The minimum inhibitory concentrations (MIC

SUBMITTER: Lin J 

PROVIDER: S-EPMC6858805 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature