Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics.

ABSTRACT: The activation of T cells by the T cell antigen receptor (TCR) results in the formation of signaling protein complexes (signalosomes), the composition of which has not been analyzed at a systems level. Here, we isolated primary CD4⁺ T cells from 15 gene-targeted mice, each expressing one tagged form of a canonical protein of the TCR-signaling pathway. Using affinity purification coupled with mass spectrometry, we analyzed the composition and dynamics of the signalosomes assembling around each of the tagged proteins over 600 s of TCR engagement. We showed that the TCR signal-transduction network comprises at least 277 unique proteins involved in 366 high-confidence interactions, and that TCR signals diversify extensively at the level of the plasma membrane. Integrating the cellul

SUBMITTER: Voisinne G 

PROVIDER: S-EPMC6859066 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature