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Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors.


ABSTRACT: Damage-induced long non-coding RNAs (dilncRNA) synthesized at DNA double-strand breaks (DSBs) by RNA polymerase II are necessary for DNA-damage-response (DDR) focus formation. We demonstrate that induction of DSBs results in the assembly of functional promoters that include a complete RNA polymerase II preinitiation complex, MED1 and CDK9. Absence or inactivation of these factors causes a reduction in DDR foci both in vivo and in an in vitro system that reconstitutes DDR events on nucleosomes. We also show that dilncRNAs drive molecular crowding of DDR proteins, such as 53BP1, into foci that exhibit liquid-liquid phase-separation condensate properties. We propose that the assembly of DSB-induced transcriptional promoters drives RNA synthesis, which stimulates phase separation of DDR factors in the shape of foci.

SUBMITTER: Pessina F 

PROVIDER: S-EPMC6859070 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors.

Pessina Fabio F   Giavazzi Fabio F   Yin Yandong Y   Gioia Ubaldo U   Vitelli Valerio V   Galbiati Alessandro A   Barozzi Sara S   Garre Massimiliano M   Oldani Amanda A   Flaus Andrew A   Cerbino Roberto R   Parazzoli Dario D   Rothenberg Eli E   d'Adda di Fagagna Fabrizio F  

Nature cell biology 20190930 10


Damage-induced long non-coding RNAs (dilncRNA) synthesized at DNA double-strand breaks (DSBs) by RNA polymerase II are necessary for DNA-damage-response (DDR) focus formation. We demonstrate that induction of DSBs results in the assembly of functional promoters that include a complete RNA polymerase II preinitiation complex, MED1 and CDK9. Absence or inactivation of these factors causes a reduction in DDR foci both in vivo and in an in vitro system that reconstitutes DDR events on nucleosomes. W  ...[more]

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