Ontology highlight
ABSTRACT:
SUBMITTER: Pessina F
PROVIDER: S-EPMC6859070 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Pessina Fabio F Giavazzi Fabio F Yin Yandong Y Gioia Ubaldo U Vitelli Valerio V Galbiati Alessandro A Barozzi Sara S Garre Massimiliano M Oldani Amanda A Flaus Andrew A Cerbino Roberto R Parazzoli Dario D Rothenberg Eli E d'Adda di Fagagna Fabrizio F
Nature cell biology 20190930 10
Damage-induced long non-coding RNAs (dilncRNA) synthesized at DNA double-strand breaks (DSBs) by RNA polymerase II are necessary for DNA-damage-response (DDR) focus formation. We demonstrate that induction of DSBs results in the assembly of functional promoters that include a complete RNA polymerase II preinitiation complex, MED1 and CDK9. Absence or inactivation of these factors causes a reduction in DDR foci both in vivo and in an in vitro system that reconstitutes DDR events on nucleosomes. W ...[more]