Quantitative analysis of T cell proteomes and environmental sensors during T cell differentiation.

ABSTRACT: Quantitative mass spectrometry reveals how CD4⁺ and CD8⁺ T cells restructure proteomes in response to antigen and mammalian target of rapamycin complex 1 (mTORC1). Analysis of copy numbers per cell of >9,000 proteins provides new understanding of T cell phenotypes, exposing the metabolic and protein synthesis machinery and environmental sensors that shape T cell fate. We reveal that lymphocyte environment sensing is controlled by immune activation, and that CD4⁺ and CD8⁺ T cells differ in their intrinsic nutrient transport and biosynthetic capacity. Our data also reveal shared and divergent outcomes of mTORC1 inhibition in naïve versus effector T cells: mTORC1 inhibition impaired cell cycle progression in activated naïve cells, but not effector c

SUBMITTER: Howden AJM 

PROVIDER: S-EPMC6859072 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature