Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.

ABSTRACT: In eukaryotes, accurate chromosome segregation in mitosis and meiosis maintains genome stability and prevents aneuploidy. Kinetochores are large protein complexes that, by assembling onto specialized Cenp-A nucleosomes^1,2, function to connect centromeric chromatin to microtubules of the mitotic spindle^3,4. Whereas the centromeres of vertebrate chromosomes comprise millions of DNA base pairs and attach to multiple microtubules, the simple point centromeres of budding yeast are connected to individual microtubules^5,6. All 16 budding yeast chromosomes assemble complete kinetochores using a single Cenp-A nucleosome (Cenp-A^Nuc), each of which is perfectly centred on its cognate centromere^7-9. The inner and outer kinetochore modules are resp

SUBMITTER: Yan K 

PROVIDER: S-EPMC6859074 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature