Synthetic control devices for gene regulation in Penicillium chrysogenum.

ABSTRACT:

Background

Orthogonal, synthetic control devices were developed for Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. In the synthetic transcription factor, the QF DNA-binding domain of the transcription factor of the quinic acid gene cluster of Neurospora crassa is fused to the VP16 activation domain. This synthetic transcription factor controls the expression of genes under a synthetic promoter containing quinic acid upstream activating sequence (QUAS) elements, where it binds. A gene cluster may demand an expression tuned individually for each gene, which is a great advantage provided by this system.

Results

The control devices were characterized with respect to three of their main components: expression of the synthetic transcrip

SUBMITTER: Mozsik L 

PROVIDER: S-EPMC6859608 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature