Sequential Windowed Acquisition of Reporter Masses for Quantitation-First Proteomics.

ABSTRACT: The standard approach for proteomic data acquisition of isobaric-tagged samples by mass spectrometry is data-dependent acquisition. This semistochastic, identification-first paradigm generates a wealth of peptide-level data without regard to relative abundance. We introduce a data acquisition concept called sequential windowed acquisition of reporter masses (SWARM). This approach performs quantitation first, thereby allowing subsequent acquisition decisions to be predicated on user-defined patterns of reporter ion intensities. The efficacy of this approach is validated through experiments with both synthetic mixtures of Escherichia coli ribosomes spiked into human cell lysates at known ratios and the quantitative evaluation of the human proteome's response to the inhibition of cullin-based

SUBMITTER: Barshop WD 

PROVIDER: S-EPMC6859794 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature