RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA.

ABSTRACT: There is a pressing need for nucleic acid-based assays that are capable of rapidly and reliably detecting pathogenic organisms. Many of the techniques available for the detection of pathogenic RNA possess one or more limiting factors that make the detection of low-copy RNA challenging. Although RT-PCR is the most commonly used method for detecting pathogen-related RNA, it requires expensive thermocycling equipment and is comparatively slow. Isothermal methods promise procedural simplicity but have traditionally suffered from amplification artifacts that tend to preclude easy identification of target nucleic acids. Recently, the isothermal SHERLOCK system overcame this problem by using CRISPR to distinguish amplified target sequences from artifactual background signal. However, this system

SUBMITTER: Abdolahzadeh A 

PROVIDER: S-EPMC6859864 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature