Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.

ABSTRACT: A fundamental goal in the biological sciences is to determine how individual cells with varied gene expression profiles and diverse functional characteristics contribute to development, physiology, and disease. Here, we report a novel strategy to assess gene expression and cell physiology in single living cells. Our approach utilizes fluorescently labeled mRNA-specific anti-sense RNA probes and dsRNA-binding protein to identify the expression of specific genes in real-time at single-cell resolution via FRET. We use this technology to identify distinct myocardial subpopulations expressing the structural proteins myosin heavy chain α and myosin light chain 2a in real-time during early differentiation of human pluripotent stem cells. We combine this live-cell gene expression analysis with det

SUBMITTER: Atmanli A 

PROVIDER: S-EPMC6861004 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature