Fenofibrate increases very-long-chain sphingolipids and improves blood glucose homeostasis in NOD mice.

ABSTRACT:

Aims/hypothesis

Sphingolipid metabolism regulates beta cell biology and inflammation and is abnormal at the onset of type 1 diabetes. Fenofibrate, a regulator of sphingolipid metabolism, is known to prevent diabetes in NOD mice. Here, we aimed to investigate the effects of fenofibrate on the pancreatic lipidome, pancreas morphology, pancreatic sympathetic nerves and blood glucose homeostasis in NOD mice.

Methods

We treated female NOD mice with fenofibrate from 3 weeks of age. The pancreatic lipidome was analysed using MS. Analysis of pancreas and islet volume was performed by stereology. Islet sympathetic nerve fibre volume was evaluated using tyrosine hydroxylase staining. The effect on blood glucose homeostasis was assessed by measuring non-fasting blood glucose from age 1

SUBMITTER: Holm LJ 

PROVIDER: S-EPMC6861358 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature