Induced pluripotent stem cell macrophages present antigen to proinsulin-specific T cell receptors from donor-matched islet-infiltrating T cells in type 1 diabetes.

ABSTRACT:

Aims/hypothesis

Type 1 diabetes is an autoimmune disorder characterised by loss of insulin-producing beta cells of the pancreas. Progress in understanding the cellular and molecular mechanisms underlying the human disease has been hampered by a dearth of appropriate human experimental models. We previously reported the characterisation of islet-infiltrating CD4⁺ T cells from a deceased organ donor who had type 1 diabetes.

Methods

Induced pluripotent stem cell (iPSC) lines derived from the above donor were differentiated into CD14⁺ macrophages and tested for their capacity to present antigen to T cell receptors (TCRs) derived from islet-infiltrating CD4⁺ T cells from the same donor.

Results

The iPSC macrophages displayed typical macroph

SUBMITTER: Joshi K 

PROVIDER: S-EPMC6861360 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature