Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients.

ABSTRACT:

Objective

To evaluate the association of miR-196a rs11614913 C/T genetic variation and its target gene annexin A1 mRNA expression with breast cancer risk in Pakistani female ethnicities.

Methods

This case control study, conducted from March 2017 to November 2018 included 295 breast cancer patients, 295 controls of three Pakistani ethnicities and archived 100 samples of cohort group for genotyping and expression profiling. Genotyping of miR-196a (rs11614913 C/T) was done by ARMS PCR technique. Annexin-A1 (ANXA1) mRNA expression was measured with qRT-PCR and detection of protein expression of ANXA1 was done by immunohistochemistry.

Results

CC homozygous genotype of miR-196a rs11614913 was present in 81.4% of cases and 73.9% controls. C

SUBMITTER: Rahim A 

PROVIDER: S-EPMC6861506 | biostudies-literature | 2019 Nov-Dec

REPOSITORIES: biostudies-literature