Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types.

ABSTRACT: Floor materials in indoor environments are known to be reservoirs of microbes. We focused on examining bacterial community composition, antibiotic resistance (AR) and microbial source tracking (MST) of fecal bacteria on the floor surfaces. Swab samples were collected from carpet and vinyl floors in three different buildings (medical, veterinary, and office buildings) from high and low traffic areas. Bacterial communities were determined with 16S rRNA sequencing, and AR (tetracycline (tetQ), sulfonamide, and carbapenem (KPC)) and MST (human-, canine-, avian-, and ruminant-specific fecal bacteria) were examined with quantitative polymerase chain reaction (PCR). The results show that Proteobacteria and Actinobacteria were the most abundant phyla. Traffic level significant

SUBMITTER: Gupta M 

PROVIDER: S-EPMC6862025 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature