Non-invasive and real-time measurement of microvascular barrier in intact lungs.

ABSTRACT: Microvascular leak is a phenomenon witnessed in multiple disease states. In organ engineering, regaining a functional barrier is the most crucial step towards creating an implantable organ. All previous methods of measuring microvascular permeability were either invasive, lengthy, introduced exogenous macromolecules, or relied on extrapolations from cultured cells. We present here a system that enables real-time measurement of microvascular permeability in intact rat lungs. Our unique system design allows direct, non-invasive measurement of average alveolar and capillary pressures, tracks flow paths within the organ, and enables calculation of lumped internal resistances including microvascular barrier. We first describe the physiology of native and decellularized lungs and the inherent pr

SUBMITTER: Engler AJ 

PROVIDER: S-EPMC6863174 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature