Protein-mediated degradation of aflatoxin B₁ by Pseudomonas putida.

ABSTRACT: Degradation or the removal of aflatoxin B₁ from agriculture commodities is very important because of its acute toxicity and economic loss due to rejection of about 25% contaminated agri produce. The present study aimed at using Pseudomonas putida for the aflatoxin B₁ (AFB₁) degradation and to understand the mechanism involved. AFB₁ degradation was studied with P. putida culture, culture supernatant, cell lysate, cell lysate in the presence of protease inhibitor, and heat-inactivated cell lysate. The remaining AFB₁ was qualitatively and quantitatively measured by thin-layer chromatography and HPLC with a UV detector. P. putida culture and culture supernatant showed 80% reduction in AFB₁ within 24 h of incubation. Cell lysate

SUBMITTER: Singh J 

PROVIDER: S-EPMC6863264 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature