Correlative light and scanning electron microscopy (CLSEM) for analysis of bacterial infection of polarized epithelial cells.

ABSTRACT: Infection of mammalian host cells by bacterial pathogens is a highly dynamic process and microscopy is instrumental to reveal the cellular and molecular details of host-pathogen interactions. Correlative light and electron microscopy (CLEM) combines the advantages of three-dimensional live cell imaging with ultrastructural analysis. The analyses of adhesion to, and invasion of polarized epithelial cells by pathogens often deploys scanning electron microscopy (SEM), since surface structures of the apical brush border can be analyzed in detail. Most available CLEM approaches focus on relocalization of separated single cells in different imaging modalities, but are not readily applicable to polarized epithelial cell monolayers, since orientation marks on substrate are overgrown during differe

SUBMITTER: Kommnick C 

PROVIDER: S-EPMC6863815 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature