Imidazole Derivatives Improve Charge Reduction and Stabilization for Native Mass Spectrometry.

ABSTRACT: Noncovalent interactions between biomolecules are critical to their activity. Native mass spectrometry (MS) has enabled characterization of these interactions by preserving noncovalent assemblies for mass analysis, including protein-ligand and protein-protein complexes for a wide range of soluble and membrane proteins. Recent advances in native MS of lipoprotein nanodiscs have also allowed characterization of antimicrobial peptides and membrane proteins embedded in intact lipid bilayers. However, conventional native electrospray ionization (ESI) can disrupt labile interactions. To stabilize macromolecular complexes for native MS, charge reducing reagents can be added to the solution prior to ESI, such as triethylamine, trimethylamine oxide, and imidazole. Lowering the charge acquired durin

SUBMITTER: Townsend JA 

PROVIDER: S-EPMC6864253 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature