Preparation of DESIGNER extracts of red clover (Trifolium pratense L.) by centrifugal partition chromatography.

ABSTRACT: Starting with an isoflavone-rich red clover extract (RCE), this study expands on the DESIGNER approach to Deplete and Enrich Select Ingredients to Generate Normalized Extract Resources using countercurrent separation (CCS) methodology. A hydrostatic CCS (also known as centrifugal partition chromatography, CPC) technique was used to enrich and deplete selected bioactive isoflavones of RCE extracts. In order to efficiently prepare large enough DESIGNER extracts from RCE for biological testing including in vivo assays, it was necessary to choose a balance between resolution and a loading capacity of at least 1 g per separation for the selected solvent system (SS). Adding 3 mL of DMSO to the sample containing equal amounts of upper and lower phases of hexanes-ethyl acetate-methanol-water (HEMW

SUBMITTER: Malca Garcia GR 

PROVIDER: S-EPMC6864275 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature