Nematicidal activity of thermostable alkaline protease produced by Saccharomonospora viridis strain Hw G550.

ABSTRACT: Application of thermostable alkaline protease to control the harmful nematodes was investigated in the current study. A total of 14 proteolytic actinomycetes were isolated from Egyptian harsh environments. Out of them, isolate G550 exhibited the highest proteolytic activity (528.9 U/ml). Protease from isolate G550 exhibited high nematicidal activity against M. incognita under laboratory conditions and caused hydrolysis of J₂S cuticle. This isolate was identified using molecular techniques and deposited in GenBank under name of Saccharomonospora viridis strain Hw G550 with accession number: MF152631. The G550 protease was extracted, characterized and applied under greenhouse conditions as nematicidal agent. This enzyme exhibited maximum activity and stability at alk

SUBMITTER: Darwesh OM 

PROVIDER: S-EPMC6864322 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature