Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.

ABSTRACT: Tissue expansion techniques physically expand swellable gel-embedded biological specimens to overcome the resolution limit of light microscopy. As the benefits of expansion come at the expense of signal concentration, imaging volume and time, and mechanical integrity of the sample, the optimal expansion ratio may widely differ depending on the experiment. However, existing expansion methods offer only fixed expansion ratios that cannot be easily adjusted to balance the gain and loss associated with expansion. Here, a hydrogel conversion-based expansion method is presented, that enables easy adjustment of the expansion ratio for individual needs, simply by changing the duration of a heating step. This method, termed ZOOM, isotropically expands samples up to eightfold in a single expansion p

SUBMITTER: Park HE 

PROVIDER: S-EPMC6864509 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature