Insights into Heterologous Biosynthesis of Arteannuin B and Artemisinin in Physcomitrella patens.

ABSTRACT: : Metabolic engineering is an integrated bioengineering approach, which has made considerable progress in producing terpenoids in plants and fermentable hosts. Here, the full biosynthetic pathway of artemisinin, originating from Artemisia annua, was integrated into the moss Physcomitrella patens. Different combinations of the five artemisinin biosynthesis genes were ectopically expressed in P. patens to study biosynthesis pathway activity, but also to ensure survival of successful transformants. Transformation of the first pathway gene, ADS, into P. patens resulted in the accumulation of the expected metabolite, amorpha-4,11-diene, and also accumulation of a second product, arteannuin B. This demonstrates the presence of endogenous promiscuous enzy

SUBMITTER: Ikram NKK 

PROVIDER: S-EPMC6864739 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature