Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma.

ABSTRACT:

Background

The existing cell surface markers used for sorting glioma stem cells (GSCs) have obvious limitations, such as vulnerability to the enzymatic digestion and time-consuming labeling procedure. Reduced nicotinamide adenine dinucleotide (NADH) as a cellular metabolite with property of autofluorescence has the potential to be used as a new biomarker for sorting GSCs.

Methods

A method for sorting GSCs was established according to the properties of the autofluorescence of NADH. Then, the NADH^high and NADH^low subpopulations were sorted. The stem-like properties of the subpopulations were evaluated by qRT-PCR, western blot analyses, limiting dilution assay, cell viability assay, bioluminescence imaging, and immunofluorescence analysis in vitro and in v

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC6865050 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature