Neutrophil elastase contributes to the pathological vascular permeability characteristic of diabetic retinopathy.

ABSTRACT:

Aims/hypothesis

Levels of neutrophil elastase, a serine protease secreted by neutrophils, are elevated in diabetes. The purpose of this study was to determine whether neutrophil elastase (NE) contributes to the diabetes-induced increase in retinal vascular permeability in mice with streptozotocin-induced diabetes, and, if so, to investigate the potential role of IL-17 in this process.

Methods

In vivo, diabetes was induced in neutrophil elastase-deficient (Elane^-/-), Il-17a^-/- and wild-type mice. After 8 months of diabetes, Elane^-/- mice and wild-type age-matched control mice were injected with FITC-BSA. Fluorescence microscopy was used to assess leakage of FITC-BSA from the retinal vasculature into the neural retina. The level of NE in Il-17a

SUBMITTER: Liu H 

PROVIDER: S-EPMC6866660 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature