Identification of upstream transcription factors and an interacting PP2C protein of dehydrin WZY2 gene in wheat.

ABSTRACT: As functional proteins dehydrins are found in many maturing seeds and vegetable tissue under adverse environmental conditions. However, the regulation of dehydrin expression remains unclear.To explore regulatory mechanisms of wheat dehydrin WZY2 expression under abiotic stresses, we constructed a cDNA library from PEG- and cold-treated wheat seedlings and performed yeast one-hybrid assay and yeast two-hybrid assay to identify the upstream transcription factors and protein interacting with dehydrin WZY2 gene. Yeast one-hybrid assay illustrated that bHLH49-like (GenBank NO. XM_020296294), zinc finger A20 and AN1 domain-containing stress-associated protein 6-like (GenBank NO. XM_020341647), and bHLH47-like (GenBank NO. XM_020313116) proteins can bind and interact with the promoter of the W

SUBMITTER: Liu H 

PROVIDER: S-EPMC6866685 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature