Oral vitamin B₁₂ supplement is delivered to the distal gut, altering the corrinoid profile and selectively depleting Bacteroides in C57BL/6 mice.

ABSTRACT: Vitamin B₁₂ is a critical nutrient for humans as well as microbes. Due to saturable uptake, high dose oral B₁₂ supplements are largely unabsorbed and reach the distal gut where they are available to interact with the microbiota. The aim of this study was to determine if oral B₁₂ supplementation in mice alters 1) the concentration of B₁₂ and related corrinoids in the distal gut, 2) the fecal microbiome, 3) short chain fatty acids (SCFA), and 4) susceptibility to experimental colitis. C57BL/6 mice (up to 24 animals/group) were supplemented with oral 3.94 µg/ml cyanocobalamin (B₁₂), a dose selected to approximate a single 5 mg supplement for a human. Active vitamin B₁₂ (cobalamin), and four B₁₂-analogues ([ADE]CN-Cba

SUBMITTER: Kelly CJ 

PROVIDER: S-EPMC6866687 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature