The Immune Protein Calprotectin Impacts Clostridioides difficile Metabolism through Zinc Limitation.

ABSTRACT: The intestines house a diverse microbiota that must compete for nutrients to survive, but the specific limiting nutrients that control pathogen colonization are not clearly defined. Clostridioides difficile colonization typically requires prior disruption of the microbiota, suggesting that outcompeting commensals for resources is critical to establishing C. difficile infection (CDI). The immune protein calprotectin (CP) is released into the gut lumen during CDI to chelate zinc (Zn) and other essential nutrient metals. Yet, the impact of Zn limitation on C. difficile colonization is unknown. To define C. difficile responses to Zn limitation, we performed RNA sequencing on C. difficile exposed to CP. In medium containing CP, C. difficile upregulated

SUBMITTER: Lopez CA 

PROVIDER: S-EPMC6867894 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature