Expression of the Biologically Active Insulin Analog SCI-57 in Nicotiana Benthamiana.

ABSTRACT: Diabetes mellitus is a growing problem worldwide; however, only 23% of low-income countries have access to insulin, and ironically it costs higher in such countries than high-income ones. Therefore, new strategies for insulin and insulin analogs production are urgently required to improve low-cost access to therapeutic products, so as to contain the diabetes epidemic. SCI-57 is an insulin analog with a greater affinity for the insulin receptor and lower thermal degradation than native insulin. It also shows native mitogenicity and insulin-like biological activity. In this work, SCI-57 was transiently expressed in the Nicotiana benthamiana (Nb) plant, and we also evaluated some of its relevant biological effects. An expression plasmid was engineered to translate an N-terminal

SUBMITTER: Munoz-Talavera A 

PROVIDER: S-EPMC6868099 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature