Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014.

ABSTRACT:

Background and aim

Newcastle disease (ND) is a worldwide poultry disease that is historically known to cause severe losses in the poultry industry. In the present study, attempts were made to characterize ND virus (NDV) recovered from broiler chickens in the Eastern Region of Saudi Arabia from January 2012 to March 2014.

Materials and methods

Reverse transcription-polymerase chain reaction was used for the detection of NDV followed by partial sequencing of the fusion (F) gene. The intracerebral pathogenicity index (ICPI), mean death time (MDT), and complete sequencing of the hemagglutinin-neuraminidase (HN) gene were also used for further biological and molecular characterization.

Results

NDV was detected at a rate of 9.6% (11/115) of the tested flocks, most of which

SUBMITTER: Almubarak AIA 

PROVIDER: S-EPMC6868251 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature