Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis.

ABSTRACT:

Aim

This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.

Materials and methods

The bacterium was isolated from preputial swabs of healthy Aceh cattle. The total DNA from the isolated bacteria was extracted using the Genomic DNA Mini Kit followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The product of PCR amplification was then sequenced and aligned to the known sequences in the GenBank database by multiple alignments and was also analyzed by bioinformatics software to construct a phylogenetic tree.

Results

The results revealed that the bacterial isolate 3A had genetically closed relation to Staphylococcus pasteuri with <97% maxi

SUBMITTER: Hambal M 

PROVIDER: S-EPMC6868254 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature