Identification of cell context-dependent YAP-associated proteins reveals β₁ and β₄ integrin mediate YAP translocation independently of cell spreading.

ABSTRACT: Yes-associated protein (YAP) is a transcriptional regulator and mechanotransducer, relaying extracellular matrix (ECM) stiffness into proliferative gene expression in 2D culture. Previous studies show that YAP activation is dependent on F-actin stress fiber mediated nuclear pore opening, however the protein mediators of YAP translocation remain unclear. Here, we show that YAP co-localizes with F-actin during activating conditions, such as sparse plating and culturing on stiff 2D substrates. To identify proteins mediating YAP translocation, we performed co-immunoprecipitation followed by mass spectrometry (co-IP/MS) for proteins that differentially associated with YAP under activating conditions. Interestingly, YAP preferentially associates with β₁ integrin under activating condi

SUBMITTER: Lee JY 

PROVIDER: S-EPMC6868278 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature