A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.

ABSTRACT: Natural organisms have evolved intricate regulatory mechanisms that sense and respond to fluctuating environmental temperatures in a heat- or cold-inducible fashion. Unlike dominant heat-inducible switches, very few cold-inducible genetic switches are available in either natural or engineered systems. Moreover, the available cold-inducible switches still have many shortcomings, including high leaky gene expression, small dynamic range (<10-fold) or broad transition temperature (>10°C). To address these problems, a high-performance cold-inducible switch that can tightly control target gene expression is highly desired. Here, we introduce a tight and fast cold-inducible switch that couples two evolved thermosensitive variants, TFts and TEVts, as well as an additional Mycoplasma florum Lon pr

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC6868347 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature