Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal.

ABSTRACT: In bacteria, the assembly factors tightly orchestrate the maturation of ribosomes whose competency for protein synthesis is validated by translation machinery at various stages of translation cycle. However, what transpires to the quality control measures when the ribosomes are produced with assembly defects remains enigmatic. In Escherichia coli, we show that 30S ribosomes that harbour assembly defects due to the lack of assembly factors such as RbfA and KsgA display suboptimal initiation codon recognition and bypass the critical codon-anticodon proofreading steps during translation initiation. These premature ribosomes on entering the translation cycle compromise the fidelity of decoding that gives rise to errors during initiation and elongation. We show that the assembly defects comprom

SUBMITTER: Sharma H 

PROVIDER: S-EPMC6868393 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature