Tumor-derived exosomal miRNA-320d as a biomarker for metastatic colorectal cancer.

ABSTRACT:

Background

To identify specific exosomal microRNAs (miRNAs) as serum biomarkers for prediction of metastasis in patients with colorectal cancer (CRC).

Materials and methods

Serum exosomes were isolated from patients with metastatic CRC (n = 34) and non-metastatic CRC (n = 108) by ultracentrifugation and characterized using transmission electron microscopy, qNano, and Western blot. Differential exosomal miRNAs were screened by sequencing and validated by qPCR in metastatic and non-metastatic CRC patients.

Results

After sequence analysis, KEGG analysis showed that differential genes were associated with Rap1 signaling pathway and pathways in cancer, 6 upregulated exosomal miRNAs (miR-224-5p, miR-548d-5p, miR-200a-3p, miR-320d, miR-200b-3p, and miR-1246), and 3 downregul

SUBMITTER: Tang Y 

PROVIDER: S-EPMC6868417 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature