US3/Rps3 controls fidelity of translation termination and programmed stop codon readthrough in co-operation with eIF3.

ABSTRACT: Ribosome was long considered as a critical yet passive player in protein synthesis. Only recently the role of its basic components, ribosomal RNAs and proteins, in translational control has begun to emerge. Here we examined function of the small ribosomal protein uS3/Rps3, earlier shown to interact with eukaryotic translation initiation factor eIF3, in termination. We identified two residues in consecutive helices occurring in the mRNA entry pore, whose mutations to the opposite charge either reduced (K108E) or increased (R116D) stop codon readthrough. Whereas the latter increased overall levels of eIF3-containing terminating ribosomes in heavy polysomes in vivo indicating slower termination rates, the former specifically reduced eIF3 amounts in termination complexes. Combining these two m

SUBMITTER: Poncova K 

PROVIDER: S-EPMC6868437 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature