Comparative transcriptomic analysis reveals beneficial effect of dietary mulberry leaves on the muscle quality of finishing pigs.

ABSTRACT:

Background

The aim of this study was to investigate the effect of dietary mulberry leaves on the transcriptome profiles of finishing pigs. RNA-Seq was used to identify differentially expressed genes (DEGs) in the longissimus dorsi of 56 pigs fed either a traditional diet or diets supplemented with 3%, 6% or 9% mulberry leaf powder, and both gene ontology (GO) function and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were performed. Furthermore, protein-protein interaction (PPI) network and the subnet module analysis were used to identify genes with beneficial potential, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression patterns revealed by RNA-Seq.

Results

Pigs fed with the 6% mulberry diet exhibi

SUBMITTER: Chen G 

PROVIDER: S-EPMC6868455 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature