A novel and translational role for autophagy in antisense oligonucleotide trafficking and activity.

ABSTRACT: Endocytosis is a mechanism by which cells sense their environment and internalize various nutrients, growth factors and signaling molecules. This process initiates at the plasma membrane, converges with autophagy, and terminates at the lysosome. It is well-established that cellular uptake of antisense oligonucleotides (ASOs) proceeds through the endocytic pathway; however, only a small fraction escapes endosomal trafficking while the majority are rendered inactive in the lysosome. Since these pathways converge and share common molecular machinery, it is unclear if autophagy-related trafficking participates in ASO uptake or whether modulation of autophagy affects ASO activity and localization. To address these questions, we investigated the effects of autophagy modulation on ASO activity in

SUBMITTER: Ochaba J 

PROVIDER: S-EPMC6868497 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature