Agarose-Based Gel-Phase Microextraction Technique for Quick Sampling of Polar Analytes Adsorbed on Surfaces.

ABSTRACT: Sampling and extraction of chemical residues present on flat or curved surfaces as well as touch-sensitive objects are challenging. Hydrogels are characterized by high mechanical flexibility and water content. Thus, they are an ideal medium for transferring water-soluble analytes from a sampled surface to the next stage of an analytical workflow. Here, we demonstrate gel-phase microextraction (GPME), in which disks of blended hydrogels are utilized to lift traces of water-soluble substances adsorbed on surfaces. The protocol has been optimized in a series of tests involving fluorometric and mass spectrometric measurements. Compared with the pure agarose hydrogel, most of the tested blended hydrogels provide a higher efficiency for the sampling/extraction of a model analyte, fluorescein. Th

SUBMITTER: Liao PH 

PROVIDER: S-EPMC6868603 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature